Disc 1
| 01 | Title | 1:25 |
| 02 | Menu Select | 1:52 |
| 03 | Prologue | 1:48 |
| 04 | Frigate Orpheon | 2:45 |
| 05 | VS. Parasite Queen | 1:36 |
| 06 | Frigate Orpheon Escape | 1:31 |
| 07 | Planet Tallon IV | 4:17 |
| 08 | Ancient Chozo Ruins | 2:17 |
| 09 | VS. Hive Totem | 1:39 |
| 10 | VS. Giant Beetle | 0:45 |
| 11 | Chozo Ruins | 4:13 |
| 12 | Energy Core | 1:20 |
| 13 | VS. Flaahgra | 2:20 |
| 14 | Lava Caves (Burning Trail) | 0:42 |
| 15 | Lava Caves | 4:46 |
| 16 | Ice Valley | 3:40 |
| 17 | Ice Chapel | 1:20 |
| 18 | Space Pirates (Laboratory Gate) | 0:58 |
| 19 | VS. Space Pirates | 1:50 |
| 20 | VS. Thardus | 1:41 |
| 21 | Tallon Overworld | 3:57 |
| 22 | Chozo Elder Cathedral | 1:13 |
| 23 | VS. Chozo Ghost | 0:57 |
| 24 | Ice Valley (Phendrana Deep Lake Area) | 4:24 |
| 25 | Crashed Ship Frigate Orpheon | 3:51 |
| 26 | Phazon Mines | 0:53 |
| 27 | Phazon Area | 0:57 |
| 28 | VS. Omega Pirate | 1:47 |
| 29 | Artifact Temple | 1:36 |
| 30 | VS. Meta Ridley | 1:18 |
| 31 | Artifact Temple ~ (Chozo Seal Release) | 0:39 |
| 32 | Impact Crater | 0:31 |
| 33 | VS. Metroid Prime (Spider) | 2:37 |
| 34 | VS. Metroid Prime (Head) | 2:46 |
| 35 | Temple Collapse ~ Epilogue | 1:03 |
| 36 | Ending Staff Roll | 3:13 |
| 37 | End Jingle | 0:13 |
| 38 | Record of Samus | 2:52 |
| 39 | Samus Appearance Jingle | 0:09 |
| 40 | Item Obtainment Jingle | 0:09 |
| 41 | Artifact Obtainment Jingle | 0:08 |
Disc 2
| 01 | Metroid Fusion Arrange Version | 5:19 |
| 02 | Title | 1:18 |
| 03 | Sector 1 (SRX) SR388 Reproduction Area | 2:51 |
| 04 | Environment Sound (Silence 1) | 0:46 |
| 05 | Environment Sound (Tension) | 0:42 |
| 06 | Environment Sound (Shock) | 0:28 |
| 07 | Head to the Huge Bio-Reactions | 1:47 |
| 08 | Tension Before a Confrontation | 0:39 |
| 09 | VS. Marukara | 1:30 |
| 10 | X Invasion Detection | 1:24 |
| 11 | Navigation Room | 0:48 |
| 12 | VS. Core-X, Birdman Statue Second Form | 1:08 |
| 13 | Environment Sound (Disquieting) | 0:38 |
| 14 | Sector 2 (TRO) Tropics Area | 1:34 |
| 15 | VS. Zazabi, Varia Core-X | 1:45 |
| 16 | Environment Sound (Silence 2) | 1:05 |
| 17 | Sector 4 (AQA) Water Level Control Area | 4:23 |
| 18 | VS. Ishtar, Gedu | 1:43 |
| 19 | Sector 3 (PYR) High Temperature Zone Area | 2:09 |
| 20 | VS. Box | 0:48 |
| 21 | Sector 6 (NOC) Darkness Area | 2:59 |
| 22 | SA-X Appearance | 0:39 |
| 23 | Sector 5 (ARC) Low Temperature Zone Area | 1:11 |
| 24 | Sector 3 Emergency Occurrence | 0:32 |
| 25 | Sector 3 Cooling Device Restoration Timer Mission | 2:44 |
| 26 | Environment Sound (Intrigue) | 0:57 |
| 27 | VS. Birdman Statue First Form | 0:47 |
| 28 | VS. Nightmare | 1:02 |
| 29 | Sector 4 (AQA) Underwater Area | 2:36 |
| 30 | VS. Neo Ridley | 0:56 |
| 31 | The Last Order | 1:16 |
| 32 | VS. SA-X | 0:36 |
| 33 | Station Escape Timer Mission | 0:56 |
| 34 | Epilogue | 1:15 |
| 35 | Ending | 3:19 |
| 36 | Appearance Jingle | 0:07 |
| 37 | Item Obtainment Jingle | 0:08 |
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